The following factors contribute to the degradation of chromatography and subsequent replacement of HPLC columns:
The injection of problematic samples and/or harsh running condition can significantly shorten column life (i.e. number of injections). To maximize column lifetime, especially with problematic samples, we recommend SecurityGuard and SecurityGuard ULTRA. Phenex syringe filters can also increase column lifetimes and improve system uptime by removing particulates from your sample prior to analysis.