Region

Should I use TFA or Formic Acid for my peptide separation on a bioZen Peptide column?

GENERAL

BIOZEN HPLC UHPLC REVERSED PHASE PHARMA

Last Updated: 4/23/2018

TFA is generally the preferred mobile phase buffer additive for reversed phase separation
of peptides and protein. While some researchers prefer to use formic acid buffer
where MS detection is used, the preference for TFA in the mobile phase relates to the
fact that TFA is a weak ion-pairing buffer.