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What is the maximum concentration of salt I can run on a bioZen SEC column?

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BIOZEN HPLC UHPLC SIZE EXCLUSION PHARMA

Last Updated: 4/23/2018

We recommend 1 M as the maximum salt concentration that should be used in the
mobile phase with bioZen SEC columns. Theoretically, a higher salt concentration
should be acceptable and would not be incompatible with the columns. However, as
salt molarity increases hydrophobic interactions increase, protein solubility decreases
and column backpressure increases.