Region

What is the most common UV wavelength to detect proteins?

GENERAL

BIOZEN HPLC UHPLC REVERSED PHASE PHARMA

Last Updated: 4/23/2018

The most common wavelength used for detection of proteins is 280 nm. Absorption
of radiation in the near UV by proteins depends on the Tyr and Trp content at this
wavelength and to a very small extent the amount of Phe and disulfide bonds.