HPLC

rezex-hplc-column

Rezex HPLC Columns

Excellent Resolution and Reproducibility for Carbohydrates and Organic Acids

Rezex carbohydrate HPLC columns achieve reproducible, accurate separations based on multiple modes of interaction. Available in 4 and 8 cross linked sulfonated styrene divinylbenzene (and multiple ionic forms (sodium, hydrogen, potassium, lead, and silver) for a wide range of selectivities. USP L17, L19, L22, L34, and L58 packings available.

Use Rezex for carbohydrate, oligosaccharide, and organic acid separations:

Drug formulation and excipient analysis
Food and beverage quality control testing
Fermentation reaction monitoring and recover testing for biofuels

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Select your ideal Rezex Column phase based on type of separation or workflow

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Carbohydrate and Organic Acid Analysis

Excellent resolution and column to column reproducibility
Easy, accurate quantitation from sharper peak shapes
Longer column lifetimes and faster run time capability from lower backpressures
Baseline separation of critical sample components due to higher efficiencies

Rezex columnsfor carbohydrate analysis utilize different ligands. Selecting the right column will allow you to achieve the best resolution for your peaks of interest.

Column Selection Guidelines:

Retention time chart
Application examples
Cross reference guide

Retention Time Chart

Counter Ion Analyte

RAM Ag+

RCM Ca+2

RNM Na+

RHM H+

RPM Pb2+

Adonitol (Ribitol)

11.54

14.93

11.10

11.11

20.15

D-Altrose

11.95

12.71

11.45

10.21

15.82

D-(-)-Arabinose

13.01

13.56

12.65

11.24

16.47

D-(+)-Digitoxose

11.90

13.82

11.39

12.59

16.47

Dulcitol

11.64

21.61

11.10

10.71

33.25

Meso-Erythritol

12.31

15.49

11.78

12.14

19.82

D-(-)- Fructose

12.05

13.65

11.78

12.14

19.82

L-(-)-Fucose

12.75

13.19

12.30

11.65

16.19

D-(+)-Galactose

11.87

11.73

11.47

10.19

14.94

Gentiobiose

8.70

8.40

7.87

10.53

D-(+)-Glucose

11.04

10.37

10.71

9.62

12.92

Inositol

12.59

13.35

12.14

9.98

18.87

D- Mannitol

11.36

17.82

10.80

10.59

24.90

D-(+)-Mannose

12.04

11.54

10.16

16.39

Melibiose

9.26

9.04

8.82

8.14

11.97

D-(+)-Melezitose

8.00

7.93

7.66

7.54*

9.94

D-(+)-Raffinose

8.10

8.16

7.76

7.88*

10.28

L-(+)-Rhamnose

11.50

12.18

11.00

10.90

14.47

D-(-)-Ribose

14.59

23.38

14.34

11.42

33.48

Salicin

18.51

17.36

14.98

26.81

D-Sorbitol

11.91

22.45

11.39

10.83

35.97

Application Examples and Cross Reference Guide

Rezex

Description

Applications

Bio-Rad^® Aminex^®

Supelco^®Supelcogel™

WatersSugar-Pak^™

Transgenomic^®CARBOSep^™

Sepax^®Carbomix^®

RCM-Monosaccharide
(L19 packing)*

8 % cross-linked
CALCIUM

Monosaccharides and sugar alcohols, including sorbitol and mannitol from sweeteners and corn and cane sugars

Class separation of di-, tri-, and tetra- saccharides

HPX-87C
125-0095

Supelcogel
Ca

Sugar-Pak 1

CARBOSep
CHO-820

Carbomix Ca

RHM-Monosaccharide
(L17 packing)*

8 % cross-linkedHYDROGEN

Monosaccharides in combination with organic acids, fatty acids, alcohols, ketones,
neutral compounds, or inorganic salts

HPX-87H
125-0140

Supelcogel
C-610H & H

ICSep
ION-300

Column8

RAM-Carbohydrate

8 % cross-linked
silver

Selectivity complementary to toher Rezex column types

N/A

RSO-Oligosaccharide

4 % cross-linked silver

High resolution of oligosaccharides up to 18 degrees of polymerization (Dp)

HPX-42A
125-0097

Supelcogel
AG1 & AG2

N/A

RNO-Oligosaccharide

4 % cross-linked sodium

High resolution of oligosaccharides

N/A

CARBOSep COREGEL-87N

N/A

RPM-Monosaccharide
(L34 packing)*

8 % cross-linked LEAD

100 x 7.8 mm dimensions available for sugar alcohol analysis according to the USP procedure
Monosaccharides and sugar alcohol analysis
Cellobiose, glucose, xylose, arabinose, and mannose and other cellulose products

HPX-87P 125-0098

Supelcogel Pb

N/A

CARBOSep COREGEL-87P

Carbomix Pb

RNM-Carbohydrate

(L58 packing)*

8 % cross-linked SODIUM

Analysis of mono-, di-,and tri- saccharides
For matrices which contain high concentration of inorganic sodium (i.e. molasses)

HPX-87N
125-0143

N/A

Carbomix Na

ROA-Organic Acid

(L22 packing)*

8 % cross-linked HYDROGEN

Organic acids alone
Organic acids in combination with carbohydrates, alcohols, fatty acids, or neutral compounds
Ethanol, acetic acid, glycerol, and standard alcohol mixtures
Amino sugars

HPX-87H
125-0140

Supelcogel
C-610H & H

N/A

RFQ-Fast Acid

8 % cross-linked HYDROGEN