Region

Oligonucleotide

Purification Products

Clarity HPLC Information


Optimized Oligo Purification and Analysis

  • RPC, HPLC, prep LC, desalting, and extraction solutions
  • DNA, RNA/RNAi, longmers, dye-labeled, and modified oligonucleotides
  • High efficiency LC-MS protocols for characterization/QC
  • Personalized technical support and customer service

Each product in the Clarity BioSolutions portfolio has been designed to efficiently and effectively purify or characterize synthetic oligonucleotides used in biological research, therapeutic development and biochemical manufacturing.

Clarity BioSolutions Product Selection

Purification

  Clarity QSP™ Clarity Oligo-RP™
Clarity Oligo-XT
Clarity Oligo-SAX™ Clarity RP-Desalting™
Primary Use High-throughput, trityl-on RPC
purification
RP-HPLC purification of failure
sequences from target sequences
Economical, high loading capacity
IEX-LC prep-scale purification
Quick removal or salt & excess reagent
Purities >90% >90% >90% ~70%
Recoveries ~90% ~70% >90% ~70%
Synthesis Scale Load Up to 50 µmol Up to 50 µmol Up to 50 µmol Up to 1 µmol
Oligo Types DNA, RNA/RNAi, Thioates, Dye-labled, Modified

Characterization / Analysis

  Clarity Oligo-RP™ Clarity Oligo-MS™
Clarity Oligo-XT
Clarity Oligo-OTX™
Primary Use RP-LC-MS analysis with optimized
selectivity and sensitivity
Rapid, high efficiency RP-LC-MS
analysis for QC and characterization
Extraction of oligo therapeutics from
biological samples for LC-MS bioanalysis
Oligo Length ≤ 60 mer ≤ 60 mer ≤ 40 mer
Recommended Mobile Phase TEA / HFIP TEA/HFIP/MeOH N/A

Material Characteristics

Clarity Products Particle
Support
Bonded
Phase
Particle
shape/Size
(µm)
Pore Size
(Å)
Surface
Area
(m2/g)
Clarity QSP™ Cartridges Polymer (PSDVB) Hydrophilic polymer coating 35 500 300
Clarity Oligo-RP™
LC Columns
TWIN
(silica, organic, composite)
C18 3, 5, 10 110 375
Clarity Oligo-WAX™
LC Columns
Silica Crosslinked polyamine (WAX) 10 360
Clarity RP-Desalting™ Tubes Silica C18 55 140 300
Clarity Oligo-MS™
LC Columns
Core-Shell C18 1.7, 2.6, 5 100 200*
(*effective)
Clarity OTX™
Extraction Plates
Polymer (surface
modified PSDVB)
Mixed-mode anion exchanger 33 85 800
Clarity Oligo-SAX
LC Columns
Polymer (surface
modified PSDVB)
Hydrophilic quaternary amine 5
Clarity Oligo-XT
LC Columns
Core-Shell C18 1.7, 2.6, 5 100 200

Oligo-RP


Particle Sizes (µm)3, 5, 10
Pore Size (Å)110
Compare All Phases

Oligo-RP Phase Information


Analytical LC and Purification Solutions for Oligonucleotide

Clarity Oligo-RP columns have been specifically designed for the reversed phase purification of oligonucleotides with balanced hydrophobicity and polar selectivity. Available in 3, 5, and 10 μm particles, Clarity Oligo-RP allows for high capacity analysis and purification of oligos. Clarity Oligo-RP columns also allow for high loadability and deliver high recovery and purity, from HPLC to Preparative Purification.

A Highly Selective Analysis

Clarity Oligo-RP successfully separates a 40mer from a 39mer DNA oligonucleotide due to its excellent efficiency and resolving power.

Easily Scale from HPLC to Preparative Purification

PhaseParticle Sizes (µm)Pore Size (Å)Recommended Use
Oligo-MS1.7, 2.6100Rapid LC/MS analysis and QC of synthetic oligonucleotide research reagents and therapeutics
Oligo-RP3, 5, 10110Purification of synthetic oligonucleotide mixtures
Oligo-SAX5N/A
Oligo-WAX10400Preparative purification of synthetic oligonucleotide mixtures
Oligo-XT1.7, 2.6, 5100
Document Type:
Document Type Title
Technical Notes Analysis of Synthetic Oligonucleotides using Quaternary Amine Functionalized, Non-porous Particles - Clarity Oligo-SAX (TN-1206) Add to My Documents
Technical Notes Automated Method Development of Oligonucleotide in Tissues Using Clarity OTX 96-Well Plate and High Resolution Mass Spectrometry (TN-0097) Add to My Documents
Brochures Axia- The Ultimate Pre-packed Preparative Column Add to My Documents
Brochures Clarity Biosolutions for Synthetic DNA/RNA Add to My Documents
Brochures Improve Your Bioanalytical Assays Today Add to My Documents
Other Ultimate Guide to Reversed Phase HPLC/UHPLC - Column Selectivity Selection Poster Add to My Documents
Technical Notes Avoiding Depurination During Trityl-on Purification (TN-0008) Add to My Documents
Brochures Bulk Media Selection Guide Add to My Documents
Technical Notes Oligonucleotide Therapeutics from Biological Matrices (TN-0048) Add to My Documents
Technical Notes Optimization of Mobile Phase Composition for the Analysis of Synthetic Oligonucleotides using a Clarity Oligo-XT Column (TN-1200) Add to My Documents
Technical Notes Synthetic Oligonucleotides by LC/MS (TN-1052) Add to My Documents
MSDS Safety Data Sheet for Silica-Based HPLC Columns Add to My Documents
Other USP Allowable Adjustments Guide Add to My Documents
Supporting Docs Meeting All Your PREP Needs Add to My Documents
Other Protein and Peptide Users Guide Add to My Documents
Supporting Docs HPLC Column Care Guide Add to My Documents
Phase: Particle Size: Pore Size: Length: Internal Diameter:
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Part Number:00F-4445-P0-AX

Description:Clarity® 10 µm Oligo-RP, LC Column 150 x 21.2 mm, AXIA™ Packed, Ea

Required Holder:

Stationary Phase:C18

Solid Support:Fully Porous Organo-silica

Format:AXIAL Compression

Separation Mode:Reversed Phase

Recommended Use:Purification of synthetic oligonucleotide mixtures

USP Designation:L1


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