bioZen LC Columns

for Biologics Analysis

bioZen HPLC Information

  • Explore a New State of Zen for:
  • Peptide Mapping Aggregate Analysis Glycan Analysis Peptide Quantitation Drug Antibody Ratio Intact Mass Intact and Fragment Analysis
Ti Biocompatible Titanium Frit
Traditional Stainless Steel
Ti Biocompatible Titanium

Introducing Phenomenex bioZen HPLC/UHPLC Columns

With a new titanium hardware to minimize priming, three particle platforms for optimal versatility and eight particle chemistries to maximize selectivity and sensitivity, bioZen UHPLC/HPLC columns are seamlessly designed to bring peace of mind to your

  • Aggregate Analysis

    Quantitation of aggregate is a critical quality attribute in biopharmaceutical development. The determination of monomer, dimer, and multimer, as well as higher order structure (quaternary structural determination of a mAb), and high/low molecular weight species are commonly observed through size exclusion chromatography.

  • Drug Antibody Ratio (DAR)

    The average drug-to-antibody ratio (DAR) is critical to assess in the development of antibody-drug conjugates (ADCs). Though different chromatography modes can be employed to determine DAR, a highly effective method utilized reversed phase chromatography.

  • Glycan Analysis

    Glycosylation of proteins-specifically monoclonal antibodies-have indications on protein structure and is arguably the most critical post-translational modification to characterize in biopharmaceutical development. N-glycan analysis through hydrophilic interaction chromatography (HILIC) is a robust method for absolute quantitation of glycoforms and is the gold standard for glycosylation characterization.

  • Intact Mass

    Intact mass analysis involves using high resolution accurate mass (HRAM) instrumentation to confirm protein sequence and identify post-translational modifications. Often in combination with reversed phase chromatography, intact mass analysis is utilized on native proteins, mAbs, and ADCs in biopharmaceutical development.

  • Intact and Fragment Analysis

    Observation of intact protein and fragmented protein during impurity profiling is essential in biologic development. Utilizing reversed phase chromatography on native protein and partially fragmented protein scientists can observe characteristics including heavy chain/light chain, Fc/Fab, or isoforms.

  • Peptide Mapping

    For protein characterization, peptide mapping is commonly used for identification of non-enzymatic, spontaneous post translational modifications, such as deamidation, oxidation, succinimide formation, pyroglutamate formation, and others. Reversed phase chromatography is used for confirming primary sequence, but is more commonly for PTM identification.

  • Peptide Quantitation

    The determination of signature peptide concentration from biological matrices using LC-MS. Scientists utilize peptide quantitation to deconvolute complex chromatograms by searching for specific peptide sequences, which can analyze the peptides with high sensitivity and specificity, even at low levels of peptide.

  • Charge Variant Analysis (IEX)

    Separate MABs from from acidic/basic variants .

  • Whether you are working with
  • novel proteins

    Complex large molecules composed of amino acid polymer chains. Proteins in biopharmaceuticals typically range from 3,000-150,000 kDa. Under the biologics classification, biopharmaceutical proteins can be hormones, human growth factors, and monoclonal antibodies (mAbs). The most common biopharmaceutical proteins are insulin and mAbs.

  • or biosimilars

    A biologic with the same sequence and similar function to an innovator biologic. The characterization of biosimilars requires the same chromatographic techniques as its corresponding innovator biologic

  • bioZen UHPLC columns are guaranteed
  • to help you achieve a new
  • state of Zen for your mind, body, soul, and lab.

Zen for the Mind

Thoughtfully Designed Technologies

  • Monosized Polymeric Non-Porous, thermally modified fully porous and core-shell technology particles offer unparalleled stability, robustness, and efficiency
  • Simplified selection for intact, peptide, glycan, and size exclusion methodologies
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bioZen Core-shell Technology Particle

Monosized Polymeric Non-Porous

bioZen Thermally modified fully porous

Thermally modified fully porous

bioZen Core-shell Technology Particle

Core-shell Technology Particle

Zen for the Body

Separate a Large Body of Compounds

  • Specialized phases provide optimized elution windows for glycans, ADCs, and digested mAbs
  • bioZen columns offer better peak capacities and sharper peak shapes for increased sensitivity
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Kadcyla (4 Signature Peptides)

Kadcyla - 4 Signature Peptides analyzed on bioZen UHPLC/HPLC columns

Zen for the Soul

Versatility Down to the Very Core

  • Biocompatible titanium hardware offers better recovery and reproducibility across all workflows
  • Enhanced protein characterization using RP-MS, SEC, High-Throughput-SEC, RP-UV, HILIC-FL, HILIC-MS
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bioZen Titanium  UHPLC/HPLC columns

Zen for the Lab

Complete Support For All Your Methods

  • Full team of bioseparations Zen Masters to help you with your analysis
  • bioZen HPLC/UHPLC columns QC tested to ensure performance and reproducibility
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bioZen Zen Masters Team Pics

Find Your Zen

Screening/Early Development mAb » Preclinical »

Example of bioZen at Work:

Adalimumab using bioZen 1.8 μm SEC-3

Trastuzumab using bioZen 1.8 μm SEC-3

Rituximab using bioZen 1.8 μm SEC-3

Herceptin - mcMMAF using bioZen 1.8 μm SEC-3

Excellent ADC Peak Shape!

Trastuzumab (MES Salt Gradient) using bioZen WCX

bioZen N-Glycan Clean-Up

Solid phase extraction (SPE) HILIC stationary phase that excels at retention and recovery labeled released N-glycans! Microelution format allows for streamlined processing and clean-up of small sample volumes.

View Pricing »


WCX Phase Information

Monosized Polymeric Non-Porous Particle

Meticulously controlled monosized particle technology secures incredible particle consistency that leads to improved and reliable efficiency. This innovative non-porous particle serves as the perfect backbone for complex ion-exchange chemistries and is robust enough to handle either pH and salt gradients.


Consistent Selectivity

Each monosized particle is grafted with linear polycarboxylate chains that have been controlled within a proprietary manufacturing process to ensure a consistent stationary phase length that envelops and separate proteins (especially monoclonal antibodies) from acidic/basic variants

Document Type:
Document Type Title
Technical Notes Aggregate Analysis of Monoclonal Antibodies using bioZen SEC-3 Column Add to My Documents
Brochures Biocompatible Hardware Brochure Add to My Documents
Brochures Biologics Workflow: Aggregate Analysis (bioZen SEC Columns) Add to My Documents
Supporting Docs bioZen Column Care Note Add to My Documents
Brochures bioZen FAQ Brochure Add to My Documents
Other bioZen Phase Poster Add to My Documents
Brochures bioZen Product Guide Add to My Documents
Technical Notes bioZen Tech Tip: Deglycosylation Add to My Documents
Technical Notes bioZen Tech Tip: Drug-Antibody Ratio (DAR) Add to My Documents
Technical Notes bioZen Tech Tip: Intact Mass Add to My Documents
Technical Notes bioZen Tech Tip: Loading Capacity for SEC and Reversed Phase HPLC/UHPLC Add to My Documents
Technical Notes bioZen Tech Tip: N-Glycan Analysis Add to My Documents
Technical Notes bioZen Tech Tip: Organic Solvent and Size Exclusion Add to My Documents
Technical Notes bioZen Tech Tip: Peptide Mapping Add to My Documents
Technical Notes bioZen Tech Tip: Size Exclusion and a Well Salted Buffer Add to My Documents
Other bioZen WCX vs. Thermo ProPac WCX-10 Flyer Add to My Documents
Technical Notes Charge Variant Analysis of Cetuximab under pH and Salt Gradients using a bioZen 6 μm WCX Column Add to My Documents
Technical Notes Improved Fragment Separation using bioZen™ 1.8 μm SEC-3 (TN-1246) Add to My Documents
Technical Notes Ion-Exchange Chromatography for Charge Variant Analysis of Trastuzumab under pH and Salt Gradients using a bioZen 6 μm WCX Column Add to My Documents
Technical Notes Large vs. Small Molecules in Biopharmaceuticals Add to My Documents
Technical Notes Optimizing Monoclonal Antibody Separation using bioZen™ SEC-3 Column Add to My Documents
Technical Notes Optimizing Separation for Charge Variant Analysis (CVA) using bioZen 6 μm WCX: Salt versus pH Gradients (TN-1245) Add to My Documents
Technical Notes Released N-Glycan Analysis of Infliximab-dyyb including Sialylated Glycans using a bioZen 2.6 μm Glycan Column Add to My Documents
Technical Notes Quantitation of Neutral and Sialylated Glycans using bioZen HILIC Chromatography and Solid Phase Extraction (TN-0133) Add to My Documents
Technical Notes Sample Clean-Up After N-Linked Glycan Release and Labeling Using bioZen N-Glycan Clean-Up HILIC SPE Microelution 96-Well Plate Add to My Documents
Supporting Docs bioZen N-Glycan Workflow Solutions Add to My Documents
Phase: Particle Size: Pore Size: Length: Internal Diameter:

Part Number:00G-4777-E0

Description:bioZen™ 6µm WCX, LC Column 250 x 4.6 mm, Ea

Required Holder:

Stationary Phase:

Solid Support:


Separation Mode:

Recommended Use:

USP Designation:

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