Biosep GFC/SEC Columns

BioSep-SEC-S HPLC Information

Proteins & Peptides Simplified

BioSep™ Gel Filtration (GFC/SEC) columns are used to analyze and/or characterize proteins, peptides, and other biomolecules, including antibodies, immunoglobulins, protein complexes, protein aggregates, and desalting. BioSep columns offer many important benefits to keep your research, method development/validation, and ongoing size exclusion separations SIMPLE.

  • High Efficiencies for Greater Resolution
  • Expanded Resolution Windows
  • Excellent Reproducibility
  • Highly Inert for Better Recovery and Quantitation


Particle Sizes (µm)5
Pore Size (Å)500
Exclusion Limit1 x 10^6
USP Designation
Back Pressure (PSI) Max1500
Compare All Phases

SEC-s4000 Phase Information

  • Ideal for large proteins and PEGylated IgG.
  • Exclusion Range: 15,000 - 1,500,000 Da (Native), 15,000 - 500,000 Da (0.5% SDS), and 5,000 - 700,000 Da (6M GnHCl)
  • Resin Type: Silica
  • Particle Size: 5 µm
  • Pore Size: 500 Å
  • pH Range: 2.5 - 7.5
  • Maximum Backpressure: 1,500 psi
  • Typical Backpressure: 700 psi
  • Efficiency: 25,000 plates
PhaseParticle Sizes (µm)Pore Size (Å)Exclusion LimitUSP Design
Back Pressure
Recommended Use
SEC-s200051503 x 10^5L33, L351500Separation of biogenerics, peptide therapeutics, PEGylated peptides and peptide/protein mixtures of 1 - 300 kDa
SEC-s300052907 x 10^5L331500Separation of immunoglobulins, serum proteins, and peptide/protein mixtures of 5 - 700 kDa
SEC-s400055001 x 10^6 1500Separation of PEGylated IgG and protein mixtures of 15 - 1,500 kDa
Phase: Particle Size: Pore Size: Length: Internal Diameter:

Part Number:00H-2147-P0

Description:BioSep™ 5 µm SEC-s4000 500 Å, LC Column 300 x 21.2 mm, Ea

Required Holder:

Stationary Phase:500 Å

Solid Support:Hydrophilic Bonded Silica

Format:Preparative Column

Separation Mode:Gel Filtration Chromatography (GFC)

Recommended Use:Separation of PEGylated IgG and protein mixtures of 15 - 1,500 kDa

USP Designation:

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