Biosep GFC/SEC Columns

BioSep-SEC-S HPLC Information

Proteins & Peptides Simplified

BioSep™ Gel Filtration (GFC/SEC) columns are used to analyze and/or characterize proteins, peptides, and other biomolecules, including antibodies, immunoglobulins, protein complexes, protein aggregates, and desalting. BioSep columns offer many important benefits to keep your research, method development/validation, and ongoing size exclusion separations SIMPLE.

  • High Efficiencies for Greater Resolution
  • Expanded Resolution Windows
  • Excellent Reproducibility
  • Highly Inert for Better Recovery and Quantitation


Particle Sizes (µm)5
Pore Size (Å)290
Exclusion Limit7 x 10^5
USP DesignationL33
Back Pressure (PSI) Max1500
Compare All Phases

SEC-s3000 Phase Information

  • Ideal for immunoglobulin aggregate applications, medium to large proteins, and serum proteins.
  • Exclusion Range: 5,000 - 700,000 Da (Native), 5,000 - 100,000 Da (0.5% SDS), and 1,000 - 150,000 Da (6M GnHCl)
  • Resin Type: Silica
  • Particle Size: 5 µm
  • Pore Size: 290 Å
  • pH Range: 2.5 - 7.5
  • Maximum Backpressure: 1,500 psi
  • Typical Backpressure: 800 psi
  • Efficiency: 30,000 plates
PhaseParticle Sizes (µm)Pore Size (Å)Exclusion LimitUSP Design
Back Pressure
Recommended Use
SEC-s200051503 x 10^5L33, L351500Separation of biogenerics, peptide therapeutics, PEGylated peptides and peptide/protein mixtures of 1 - 300 kDa
SEC-s300052907 x 10^5L331500Separation of immunoglobulins, serum proteins, and peptide/protein mixtures of 5 - 700 kDa
SEC-s400055001 x 10^6 1500Separation of PEGylated IgG and protein mixtures of 15 - 1,500 kDa
Phase: Particle Size: Pore Size: Length: Internal Diameter:

Part Number:03Q-2146-K0

Description:BioSep™ 5 µm SEC-s3000 400 Å, LC Guard Column 35 x 7.8 mm, Ea

Required Holder:

Stationary Phase:290 Å

Solid Support:Hydrophilic Bonded Silica

Format:Guard Column ( conventional )

Separation Mode:Gel Filtration Chromatography (GFC)

Recommended Use:Separation of immunoglobulins, serum proteins, and peptide/protein mixtures of 5 - 700 kDa

USP Designation:L33

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