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Complementary Selectivity of bioZen™ 1.7 μm XB-C18 Peptide and bioZen 1.6 μm PS-C18 Peptide Columns for Improved Peptide Mapping (TN-1256)


Abstract:
Sequence confirmation is an important conforms to standard” criteria for testing. Monoclonal antibodies are tryptically digested to its smaller, peptidic substituents to more easily see the sequence. A key component of sequence variant analysis is sequence coverage. Though enzymatic digestion, such as with trypsin, can allow us to see more predictable peptide pieces, there are still some challenging groups of peptide pieces for analysis. Smaller, peptide pieces, for example, can be difficult to retain. Larger peptide pieces on the other hand retains well but can be challenging when it comes to ionization when using MS detection. This tech note demonstrates the use of modified C18 phases as complementary selectivities for the better retention of typically early eluting pieces and good separation of later eluting peptide pieces under mass spectrometry conditions.
Document Type:
Technical Notes
Industries:
Pharmaceutical/Biopharmaceutical
Techniques:
HPLC / UHPLC
Brands:
bioZen
PDF Link:
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