Applications

Food Additives

Analytes

1Saccharin
2Aspartame
3p-Hydroxybenzoic acid
4Caffeine
5Benzoic acid
6p-Hydroxybenzoic acid methyl ester
7p-Toluic acid
8Dehydroacetic acid
9p-Hydroxybenzoic acid ethyl ester
10p-Hydroxybenzoic acid propyl ester

Compound Name Saccharin
CID 5143
Molecular Formula C7H5NO3S
Molecular Weight 183
No. Hydrogen Bond Acceptors 3
No. Hydrogen Bond Donors 1

Details

LC Conditions (App ID: 10257)

Column:

Prodigy 5 µm Phenyl-3 (PH-3) 100 Å, LC Column 150 x 4.6 mm, Ea

Brand Name:

Prodigy

Part No:

00F-4298-E0

Phase Name:

Phenyl-3 (PH-3)

Elution Type:

Isocratic

Mobile Phase:

A: Acetonitrile

B: 20 mM Potassium phosphate pH 3.0

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	25	75

Flow Rate:

1.5 mL/min

Temperature:

40°C

Detection:

UV-Vis Abs.-Variable Wave.(UV) @ 230 nm (22°C)

Detector Info:

Variable wavelength UV detector was changed during the run as follows: 0 to 1.6 min UV @ 230 nm 1.6 to to 2 min UV @ 210 nm >2 min UV @ 230 nm

Sample Note:

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Phenyl 4 x 3.0mm, 10/Pk Part No.: AJ0-4351 - Holder Part No.: KJ0-4282

Order Items Used in This Application

00F-4298-E0

Prodigy 5 µm Phenyl-3 (PH-3) 100 Å, LC Column 150 x 4.6 mm, Ea

Qty:

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