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Applications
Chelating Agent
1285
Separation Mode: Reversed Phase
Reversed Phase
C18(2)
HPLC
Anticoagulants
Pharmaceutical
Therapeutic / Clinical

Applications

Chelating Agent
Analytes
1EDTA
Compound Name
Edetic Acid
CID
6049
Molecular Formula
C10H16N2O8
Molecular Weight
292
No. Hydrogen Bond Acceptors
10
No. Hydrogen Bond Donors
4
Details
LC Conditions (App ID: 1285)
Column:
Brand Name:
Part No:
Phase Name:
C18(2)
Elution Type:
Isocratic
Mobile Phase:
A: 72% Tetrabutylammoniom hydroxide, 0.083M, pH 3.9 with phosphoric acid
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
107228
Flow Rate:
1.2 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282

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