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Applications
Cyano Comparisons - Rhodamine Dyes
12953
Separation Mode: Normal Phase
Normal Phase
CN
HPLC
Antineoplastic Agents
Laboratory-related
Chemical Compound

Applications

Cyano Comparisons - Rhodamine Dyes
Analytes
1Rhodamine 123
2Rhodamine B
3Rhodamine 6G
Compound Name
Rhodamine 123
CID
65217
Molecular Formula
C21H17ClN2O3
Molecular Weight
380
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
2
Details
LC Conditions (App ID: 12953)
Column:
Brand Name:
Part No:
Phase Name:
CN
Elution Type:
Isocratic
Mobile Phase:
A: 80:20 20 mM Potassium phosphate pH 3.0:Methanol
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
105050
Flow Rate:
1 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 210 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, CN 4 x 3.0mm, 10/Pk Part No.: AJ0-4305 - Holder Part No.: KJ0-4282

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