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Applications
Morphine and metabolites on Synergi Hydro-RP
14238
Separation Mode: Reversed Phase
Reversed Phase
Hydro-RP
HPLC
Analgesics, Opioid
Pharmaceutical
Therapeutic / Clinical

Applications

Morphine and metabolites on Synergi Hydro-RP
Analytes
1Morphine-3-beta-D-glucuronide
2Normorphine
3Morphine
Compound Name
Morphine-3-Glucuronide
CID
5484731
Molecular Formula
C23H27NO9
Molecular Weight
461
No. Hydrogen Bond Acceptors
10
No. Hydrogen Bond Donors
5
Details
LC Conditions (App ID: 14238)
Column:
Brand Name:
Part No:
Phase Name:
Hydro-RP
Elution Type:
Gradient
Mobile Phase:
A: Water with 5mM TFA
B: Acetonitrile with 5mM TFA
Gradient Profile:
Step No.Time(min)%A%B
101000
231000
3155050
Flow Rate:
1.5 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 210 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, AQ C18 4 x 3.0mm, 10/Pk Part No.: AJ0-7511 - Holder Part No.: KJ0-4282

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