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Applications
St. John's Wort on Synergi Polar-RP
14247
Separation Mode: Reversed Phase
Reversed Phase
Polar-RP
HPLC
Antineoplastic Agents
Nutraceutical
Biochemical Compound / Nutrient

Applications

St. John's Wort on Synergi Polar-RP
Analytes
1Pseudohypericin
2Hypericin
Compound Name
Pseudohypericin
CID
5281751
Molecular Formula
C30H16O9
Molecular Weight
520
No. Hydrogen Bond Acceptors
9
No. Hydrogen Bond Donors
7
Details
LC Conditions (App ID: 14247)
Column:
Brand Name:
Part No:
Phase Name:
Polar-RP
Elution Type:
Isocratic
Mobile Phase:
A: 100mM Ammonium acetate
B: Acetonitrile
C: Methanol
Gradient Profile:
Step No.Time(min)%A%B%C
10205030
Flow Rate:
1.5 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 590 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, POLAR-RP 4 x 3.0mm, 10/Pk Part No.: AJ0-6076 - Holder Part No.: KJ0-4282

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