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Applications
Alpha and beta-acids in hop extract
14313
Separation Mode: Reversed Phase
Reversed Phase
C18(2)
HPLC
Food & Beverage
Product / Trade Good
Biochemical Compound / Nutrient

Applications

Alpha and beta-acids in hop extract
Analytes
1Cohumulone
2Ad+Humulone
3Colupulone
4Ad+Lupulone
Compound Name
Cohumulone
CID
196915
Molecular Formula
C20H28O5
Molecular Weight
348
No. Chiral Centers:
1
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
3
Details
LC Conditions (App ID: 14313)
Column:
Brand Name:
Part No:
Phase Name:
C18(2)
Elution Type:
Isocratic
Mobile Phase:
A: Methanol with 0.1% H3PO4
B: Water with 0.1% H3PO4
Gradient Profile:
Step No.Time(min)%A%B
109010
Flow Rate:
1.5 mL/min
Temperature:
30°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 314 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282

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