Applications

Gradient Optimization (2) on Jupiter Proteo using Tryptic digest of Beta-Lactoglobulin

Analytes

1beta-Lactoglobulin

Compound Name Cid9988076
CID 9988076
Molecular Formula C37H64N12O8S
Molecular Weight 837
No. Hydrogen Bond Acceptors 13
No. Hydrogen Bond Donors 10

Details

LC Conditions (App ID: 14380)

Column:

Jupiter 4 µm Proteo 90 Å, LC Column 250 x 4.6 mm, Ea

Brand Name:

Jupiter

Part No:

00G-4396-E0

Phase Name:

Proteo

Elution Type:

Gradient

Mobile Phase:

A: 1.25mM TFA in Water

B: 1.0mM TFA in Acetonitrile

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	97	3
2	10	97	3
3	90	60	40

Flow Rate:

1 mL/min

Temperature:

22°C

Detection:

UV-Vis Abs.-Variable Wave.(UV) @ 210 nm (22°C)

Sample Note:

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074 - Holder Part No.: KJ0-4282

Order Items Used in This Application

00G-4396-E0

Jupiter 4 µm Proteo 90 Å, LC Column 250 x 4.6 mm, Ea

Qty:

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