| 1IgG |
|---|
Analyte not found
Applications
IgG_Dog (Native) Jupiter 300 C4
14908
Separation Mode: Reversed Phase
Reversed Phase
C4
HPLC
Pharmaceutical/Biopharmaceutical
Life Science
Protein
Biochemical Compound / Nutrient
Analytes
Details
LC Conditions (App ID: 14908)
Column: | ||||||||||||
Brand Name: | ||||||||||||
Part No: | ||||||||||||
Phase Name: C4 | ||||||||||||
Elution Type: Gradient | ||||||||||||
Mobile Phase: A: 0.1%TFA/95% Water/ 5% Acetonitrile B: 0.085% TFA/75% Acetonitrile/ 20% IPA/5% Water | ||||||||||||
Gradient Profile:
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Flow Rate: 0.25 mL/min | ||||||||||||
Temperature: 22°C | ||||||||||||
Detection: UV-Vis Abs.-Diode Array (PDA) (22°C) | ||||||||||||
Sample Note: These applications demonstrate the utility of using low amounts of isopropanol in the organic mobile phase to improve peak shape of hydrophobic proteins. App ID#14918 shows the elution of Ig-G on a Jupiter 300 C4 column where Acetonitrile is used as the organic mobile phase. App ID#14908 shows the same Ig-G run on the same column using an organic mobile phase that contains 20% isopropanol. Note the dramatic improvement in both recovery and peak shape for the Ig-G peak. The addition of isopropanol to the organic mobile phase improves the efficiency as well as the peak shape for large hydrophobic proteins due much in part to the increased hydrophobicity of the organic mobile phase upon addition of isopropanol. Especially when performing separations of Ig-G and other immunoglobin one should start with the use of a wide pore C4 column and look at using significant amounts of IPA as part of the organic mobile phase. |
Order Items Used in This Application
00F-4167-B0
Jupiter 5 µm C4 300 Å, LC Column 150 x 2 mm, Ea
Qty:
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