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Applications
PEGylated Insulin on Jupiter® 300 C4, C18 and Two Experimental Columns
16193
Separation Mode: Reversed Phase
Reversed Phase
C4
HPLC
Pharmaceutical/Biopharmaceutical
Life Science
Biopharmaceutical
Therapeutic / Clinical

Applications

PEGylated Insulin on Jupiter® 300 C4, C18 and Two Experimental Columns
Analytes
1PEGylated Insulin

Analyte not found

Details
LC Conditions (App ID: 16193)
Column:
Brand Name:
Part No:
Phase Name:
C4
Elution Type:
Gradient
Mobile Phase:
A: 0.1% TFA and 2% ACN in Water
B: 70/20% ACN/IPA, 0.08% TFA in Water
Gradient Profile:
Step No.Time(min)%A%B
108515
2253070
Flow Rate:
1 mL/min
Temperature:
45°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (22°C)
Sample Note:
Reversed phase of PEGylated Insulin run on listed columns. Note the reduced resolution for the Jupiter C18 and EXP 2 columns when compared to the Jupiter C4 column. Although it is difficult to visualize, the Jupiter actully has better resolution of PEGylated forms than the EXP1 column.

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