1PEGylated Ribonuclease A |
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Analyte not found
1PEGylated Ribonuclease A |
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Analyte not found
Column: | ||||||||||||
Brand Name: | ||||||||||||
Part No: | ||||||||||||
Phase Name: C4 | ||||||||||||
Elution Type: Gradient | ||||||||||||
Mobile Phase: A: 0.1% TFA in Water B: 90% ACN 0.09% TFA in Water | ||||||||||||
Gradient Profile:
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Flow Rate: 1 mL/min | ||||||||||||
Temperature: 45°C | ||||||||||||
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (22°C) | ||||||||||||
Sample Note: Reversed phase runs of PEGylated ribonuclease A using different gradient conditions on a Jupiter 300 C4 column (gradient length 25 min). Note that if initial %B is increased much above 25% then retention and resolution of PEGylated forms is lost. Gradient slopes of between 1-2%/min appear to be a good compromise between resolution and run time. |