Applications

Jupiter® 300 C4 - PEGylation Reaction Time Course (Ribonuclease A)

Analytes

1PEGylated Ribonuclease A

Analyte not found

Details

LC Conditions (App ID: 16199)

Column:

Jupiter 5 µm C4 300 Å, LC Column 150 x 4.6 mm, Ea

Brand Name:

Jupiter

Part No:

00F-4167-E0

Phase Name:

Elution Type:

Gradient

Mobile Phase:

A: 0.1% TFA in Water

B: 90% ACN 0.09% TFA in Water

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	80	20
2	25	45	55

Flow Rate:

1 mL/min

Temperature:

45°C

Detection:

UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (22°C)

Sample Note:

PEGylation time course study of ribonuclease. Different time points from a PEGylation reaction of ribonuclease A were taken: unreacted, 0 min., 3 min., 9 min., 30 min., 90 min., and 120 min. Note the increase of greater retaining PEGylation forms over time.

Order Items Used in This Application

00F-4167-E0

Jupiter 5 µm C4 300 Å, LC Column 150 x 4.6 mm, Ea

Qty:

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