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Applications
Morphine and Metabolites on Luna 5um HILIC
16334
Separation Mode: Hydrophilic Interaction
Hydrophilic Interaction
HILIC
HPLC
Central Nervous System Stimulants
Pharmaceutical
Therapeutic / Clinical

Applications

Morphine and Metabolites on Luna 5um HILIC
Analytes
1Morphine
2Normorphine
3Morphine-3-beta-D-glucuronide
Compound Name
Morphine
CID
5288826
Molecular Formula
C17H19NO3
Molecular Weight
285
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
2
Details
LC Conditions (App ID: 16334)
Column:
Brand Name:
Part No:
Phase Name:
HILIC
Elution Type:
Gradient
Mobile Phase:
A: 90/10 Acetonitrile/100mM Ammonium Formate pH 3.2
B: 50/50 Acetonitrile/20mM Ammonium Formate pH 3.2
Gradient Profile:
Step No.Time(min)%A%B
101000
211000
330100
44.50100
54.511000
69.511000
Flow Rate:
0.4 mL/min
Temperature:
22°C
Detection:
Mass Spectrometer (MS) (22°C)
Detector Info:
ABI 3000 Triple Quad MS
Sample Note:
The pH of the aqueous buffer is specified. The pH in mixed organic was not measured.

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