For security purposes
FOR SECURITY PURPOSES - because Internet Explorer is no longer supported by Microsoft, we suggest that you interact with our secure site through one of our supported browsers - Google Chrome, Firefox, or MS Edge. If you continue to use this website with Internet Explorer you do so at your own risk and you may encounter problems.
Applications
PEGylated Ribonuclease A on Jupiter® 300 C18 3µm, reaction at different time-points
17515
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Pharmaceutical/Biopharmaceutical
Life Science
Protein
Biochemical Compound / Nutrient

Applications

PEGylated Ribonuclease A on Jupiter® 300 C18 3µm, reaction at different time-points
Analytes
1PEGylated Ribonuclease A

Analyte not found

Details
LC Conditions (App ID: 17515)
Column:
Brand Name:
Part No:
Phase Name:
C18
Elution Type:
Gradient
Mobile Phase:
A: 0.1% TFA, 2% ACN in Water
B: 90% ACN, 0.085% TFA in Water
Gradient Profile:
Step No.Time(min)%A%B
108020
2253565
Flow Rate:
1 mL/min
Temperature:
45°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (25°C)
Sample Note:
Separation of N-Terminus PEGylated Rebonuclease A with 20kDa Methoxy PEG aldehyde (PEG1) on Jupiter 300 3µm C18.

Order Items Used in This Application

Similar Applications
search
BioSep 5 µm SEC-s2000 145 Å, LC Column 300 x 7.8 mm, Ea
Gel Filtration Chromatography (GFC)
Jupiter 5 µm C4 300 Å, LC Column 150 x 4.6 mm, Ea
Reversed Phase
Jupiter 5 µm C4 300 Å, LC Column 150 x 4.6 mm, Ea
Reversed Phase
BioSep 5 µm SEC-s2000 145 Å, LC Column 300 x 7.8 mm, Ea
Gel Filtration Chromatography (GFC)
Jupiter 5 µm C4 300 Å, LC Column 150 x 4.6 mm, Ea
Reversed Phase
Jupiter 5 µm C4 300 Å, LC Column 150 x 4.6 mm, Ea
Reversed Phase