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Applications
Melamine and Cyanuric Acid on Kinetex 2.6 µm HILIC 50 x 2.1 mm with Acquity UPLC MS/MS
18723
Separation Mode: Hydrophilic Interaction
Hydrophilic Interaction
HILIC
HPLC
Food & Beverage
Pollutant / Contaminant
Environmental / Health

Applications

Melamine and Cyanuric Acid on Kinetex 2.6 µm HILIC 50 x 2.1 mm with Acquity UPLC MS/MS
Analytes
1Cyanuric acid
2Cyanuric acid-13C3
3Melamine
4Melamine-13C3.15N3
Compound Name
Isocyanuric Acid
CID
7956
Molecular Formula
C3H3N3O3
Molecular Weight
129
No. Hydrogen Bond Acceptors
3
No. Hydrogen Bond Donors
3
Details
LC Conditions (App ID: 18723)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
HILIC
Elution Type:
Gradient
Mobile Phase:
A: 90/10 ACN/100mM NH4 acetate pH 5.8
Gradient Profile:
Step No.Time(min)%A
10100
20100
Flow Rate:
1 mL/min
Temperature:
0°C
Detection:
Mass Spectrometer (MS) (110°C)
Detector Info:
Switch from Negative Ion Mode (Cyanuric acid) to Positive Ion (for Melamine) at 0.26 minutes until 1 minute; MRM Cyanuric acid 128-85.0 (quant ion),128.0-42.0 (qualifier ion), Cyanuric acid-13C3 ISTD 131.1-87.0, Melamine 127.1-85 (quant ion), 127.1-68 (qualifier ion), Melamine-13C3.15N3 ISTD 133.2-89.1

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