| 1Androsterone |
|---|
| 2DHEA |
| 317-alpha-Estradiol |
| 4Estrone |
| 517-beta-Estradiol |
| 6Testosterone |
Compound Name Androsterone |
CID 5879 |
Molecular Formula C19H30O2 |
Molecular Weight 290 |
No. Hydrogen Bond Acceptors 2 |
No. Hydrogen Bond Donors 1 |
Applications
Sex hormones on a ZB-1ms 30x0.25x0.25 by GCMS
19293
Androgens
Clinical
Clinical Research
Therapeutic / Clinical
Analytes
Details
GC Conditions (App ID: 19293)
Injection: Split 10:1 @ 250°C, 1.0 μL |
Oven Program: 220ºC to 320ºC @ 10ºC/min for 5 min |
Carrier Gas: Constant Flow Helium, 1.0 mL/min |
Detection: Mass Selective (MSD) @ 50 amu (320°C) |
Sample Notes: Sample Prep: 50µL of 100µg/mL hormone mix in methanol is reacted with 50µL of 2% methoxyamine in pyridine @ 60ºC for 30 minutes. Reaction mixture is evaporated to dryness under nitrogen at room temp and reconstituted in BSTFA. The BSTFA mixture is reacted at 70ºC for 30 minutes and then injected into GCMS.
Note: There is slight peak splitting of testosterone due to isomerization of the oxime-TMS derivative. |
Similar Applications
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Androsterone
Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea
Application Specific
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