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Applications
Water soluble vitamins on Synergi Hydro-RP
19722
Separation Mode: Reversed Phase
Reversed Phase
Hydro-RP
HPLC
Vitamin B Complex
Food & Beverage

Applications

Water soluble vitamins on Synergi Hydro-RP
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Analytes
1Thiamine
2Nicotinic acid
3Nicotinamide
4Pyridoxine
5Pantothenic acid
6Folic acid
7Riboflavin
8Biotin
Compound Name
Thiamin
CID
1130
Molecular Formula
C12H17N4OS+
Molecular Weight
265
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
2
Total Formal Charge
1
Details
LC Conditions (App ID: 19722)
Column:
Brand Name:
Part No:
Phase Name:
Hydro-RP
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic Acid in DI H2O
B: 100% ACN
Gradient Profile:
Step No.Time(min)%A%B
10982
22982
354060
464060
56.11090
681090
78.1982
Flow Rate:
0.5 mL/min
Temperature:
0°C
Detection:
Mass Spectrometer (MS) (22°C)
Detector Info:
SCIEX API 4000™

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