| 1Phospholipid Profile |
|---|
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Applications
Phospholipid Removal Phree vs. Traditional PPT(Zoomed Scale)
20012
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Clinical
Pharmaceutical/Biopharmaceutical
Forensics/Toxicology
Analytes
Details
LC Conditions (App ID: 20012)
Column: | ||||||||||||||||||||||||
Brand Name: Kinetex | ||||||||||||||||||||||||
Part No: | ||||||||||||||||||||||||
Phase Name: C18 | ||||||||||||||||||||||||
Elution Type: Gradient | ||||||||||||||||||||||||
Mobile Phase: A: 0.1% Formic Acid in Water B: 0.1% Formic Acid in Methanol | ||||||||||||||||||||||||
Gradient Profile:
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Flow Rate: 400 µL/min | ||||||||||||||||||||||||
Temperature: 22°C | ||||||||||||||||||||||||
Detection: Mass Spectrometer (MS) @ 184 amu (425°C) | ||||||||||||||||||||||||
Sample Note: Extracted sample was prepped with Phenomenex Phree Phospholipid Removal Plate. Step 1: Add 300uL of 1% formic acid in acetonitrile directly to Phree plate. Step 2: Add 100uL of plasma directly to Phree plate. Vortex and extract. This was compared to standard protein precipitated plasma using the same volume of plasma and crash solvent |
Sample Preparation Details
Description: Phree Phospholipid Removal, 30 mg / well, 96-Well Plates, 2/Pk |
Part Number: |
Evaporation: Evaporate to dryness |
Order Items Used in This Application
00B-4462-AN
Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
Qty:
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