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Applications
MMA and Succinic Acid in Human Serum on Gemini C18, 3u, 100x3 mm
20587
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Radiation-Protective Agents
Clinical
Therapeutic / Clinical
Biochemical Compound / Nutrient

Applications

MMA and Succinic Acid in Human Serum on Gemini C18, 3u, 100x3 mm
Analytes
1Succinic acid
2Methylmalonic acid-d3
3Methylmalonic acid
Compound Name
Succinic Acid
CID
1110
Molecular Formula
C4H6O4
Molecular Weight
118
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
2
Details
LC Conditions (App ID: 20587)
Column:
Brand Name:
Part No:
Phase Name:
C18
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic Acid in DI H2O
B: 0.1% Formic Acid + 10 mM Ammonium Formate in MeOH
Gradient Profile:
Step No.Time(min)%A%B
108515
21.5595
32.5595
42.518515
54.58515
Flow Rate:
700 µL/min
Temperature:
40°C
Detection:
Tandem Mass Spec (MS-MS) (22°C)
Detector Info:
SCIEX API 4000™ (negative Mode ESI)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Gemini C18 4 x 2.0mm ID, 10/Pk Part No.: AJ0-7596 - Holder Part No.: KJ0-4282

Order Items Used in This Application

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