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Applications
Estradiol and estrone analysis by LCMS using Gemini 3um NX-C18 50x2mm
20653
Separation Mode: Reversed Phase
Reversed Phase
NX-C18
HPLC
Clinical
Biochemical Compound / Nutrient
Therapeutic / Clinical
Chemical Compound
Therapeutic / Clinical
Therapeutic / Clinical

Applications

Estradiol and estrone analysis by LCMS using Gemini 3um NX-C18 50x2mm
Analytes
1Estrone
2Estrone-D4
3Estradiol
4Estradiol-D3
5Estriol
6Estriol-D3
Compound Name
Estrone
CID
5870
Molecular Formula
C18H22O2
Molecular Weight
270
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
1
Details
LC Conditions (App ID: 20653)
Column:
Brand Name:
Part No:
Phase Name:
NX-C18
Elution Type:
Gradient
Mobile Phase:
A: H2O + 0.5 mM ammonium hydroxide
B: MeOH + 0.5 mM ammonium hydroxide
Gradient Profile:
Step No.Time(min)%A%B
109010
20.59010
32.52080
451090
55.29010
669010
Flow Rate:
0.55 mL/min
Temperature:
22°C
Detection:
Tandem Mass Spec (MS-MS) (22°C)
Detector Info:
SCIEX API 4000™

Order Items Used in This Application

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