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Applications
Biogenic Amines by LCMS using Kinetex 1.7u C18 50x3.0mm
20685
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Anti-Infective Agents, Local
Clinical
Biochemical Compound / Nutrient
Therapeutic / Clinical

Applications

Biogenic Amines by LCMS using Kinetex 1.7u C18 50x3.0mm
Analytes
1IQx
28-MeIQx
3IQ
47,8-DiMeIQx
54,8-DiMeI-Qx
6MeIQ
7Trp-P-2
8Harman
9PhIP
10Norharman
11AaC
12MeAaC
Compound Name
Molport-003-844-559
CID
105041
Molecular Formula
C10H9N5
Molecular Weight
199
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
1
Details
LC Conditions (App ID: 20685)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
C18
Elution Type:
Gradient
Mobile Phase:
A: 30mM Ammonium formate
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
108020
234060
33.10100
440100
54.18020
658020
Flow Rate:
600 µL/min
Temperature:
22°C
Detection:
Tandem Mass Spec (MS-MS) (22°C)
Detector Info:
SCIEX API 4000™
Sample Preparation Details
Description:
Strata-X-C 33 µm Polymeric Strong Cation, 60 mg / 3 mL, Tubes, 50/Pk
Part Number:
Condition 1:
Methanol
Condition 2:
Water
Wash 1:
0.1M HCl
Wash 2:
Methanol
Evaporation:
Evaporate to dryness
1
Condition
Methanol
2
Wash
Methanol
1
Condition
Methanol
1
Shake
0.2M Ammonia Solution made up in methanol
2
Condition
Water

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