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Applications
Heterocyclic Amines by LCMS using Kinetex 5u C18 50x2.1mm
20771
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Mutagens
Environmental
Food & Beverage
Environmental / Health
Product / Trade Good
Environmental / Health

Applications

Heterocyclic Amines by LCMS using Kinetex 5u C18 50x2.1mm
Analytes
1DMIP
2IQx
3IQ
48-MeIQ
54,8-DiMe-IQx
67,8-DiMe-IQx
7Trp-P-2
8Harman
9Norharman
10PhIP
11AaC
12MeAaC
13MeIQ

Analyte not found

Details
LC Conditions (App ID: 20771)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
C18
Elution Type:
Gradient
Mobile Phase:
A: 30 mM Ammonium formate
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
10955
254060
35.10100
460100
57955
Flow Rate:
500 µL/min
Temperature:
22°C
Detection:
Tandem Mass Spec (MS-MS) (22°C)
Detector Info:
SCIEX API 4000™
Sample Preparation Details
Description:
Strata-X-C 33 µm Polymeric Strong Cation, 60 mg / 3 mL, Tubes, 50/Pk
Part Number:
Condition 1:
Methanol
Condition 2:
Water
Wash 1:
0.1M HCl
Wash 2:
Methanol
Evaporation:
Evaporate to dryness
1
Condition
Methanol
2
Wash
Methanol
1
Shake
0.2M Ammonia Solution made up in methanol
1
Wash
0.1M HCl
2
Condition
Water

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