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Applications

Human Plasma Vitamin C on Kinetex 5u XB-C18, 150x4.6mm Wavelength Study

Analytes

1Vitamin C

Compound Name Ascorbic Acid
CID 5785
Molecular Formula C6H8O6
Molecular Weight 176
No. Hydrogen Bond Acceptors 6
No. Hydrogen Bond Donors 4

Details

LC Conditions (App ID: 21442)

Column:

Kinetex 5 µm XB-C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand Name:

Kinetex

Part No:

Phase Name:

XB-C18

Elution Type:

Gradient

Mobile Phase:

A: 0.1% formic acid

B: Acetonitrile 100%

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	100	0
2	3.5	100	0
3	3.6	0	100
4	5	0	100
5	5.1	100	0
6	7	100	0

Flow Rate:

800 µL/min

Temperature:

22°C

Detection:

UV-Vis Abs.-Variable Wave.(UV) @ 245 nm (22°C)

Sample Note:

Guard column and cartridges were used for human plasma vitamin C analysis: SecurityGuard Ultra Cartridges: Cat log No. AJO-8768. SecurityGuard Ultra Cartridge holder: Cat log No. AJO-9000.

Sample Preparation Details

Description: Impact Protein Precipitation, 2mL Square Well Filter Plate, 2/Pk
Part Number: CE0-7565
Evaporation: Evaporate to dryness

Order Items Used in This Application

00F-4605-E0

Kinetex 5 µm XB-C18 100 Å, LC Column 150 x 4.6 mm, Ea

Qty:

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