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Applications
PLP and PA in Human sera/plasma on Gemini-NX 3u, C18, 100x4.6 by HPLC w Fluorescence Detection-5
21885
Separation Mode: Reversed Phase
Reversed Phase
NX-C18
HPLC
Vitamin B Complex
Clinical

Applications

PLP and PA in Human sera/plasma on Gemini-NX 3u, C18, 100x4.6 by HPLC w Fluorescence Detection-5
Analytes
1Pyridoxal 5'-phosphate
24-Pyridoxic acid
3Pyridoxal
Compound Name
Codecarboxylase
CID
1051
Molecular Formula
C8H10NO6P
Molecular Weight
247
No. Hydrogen Bond Acceptors
7
No. Hydrogen Bond Donors
3
Details
LC Conditions (App ID: 21885)
Column:
Brand Name:
Part No:
Phase Name:
NX-C18
Elution Type:
Gradient
Mobile Phase:
A: 20mmol/L Na2HPO4 + 1mL acetic acid in 1L DI Water
B: ACN : MeOH (70:30)
Gradient Profile:
Step No.Time(min)%A%B
10955
254060
35.1595
46955
57955
Flow Rate:
1 mL/min
Temperature:
35°C
Detection:
Fluorescence (FLUOR) @ Ex 360 nm (22°C)
Detector Info:
Shimadzu RF-20A

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Reversed Phase