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Applications
Nicotines at High pH using Kinetex 5u EVO C18 100x3.0mm
22721
Separation Mode: Reversed Phase
Reversed Phase
EVO C18
HPLC
Nicotinic Agonists
Pharmaceutical/Biopharmaceutical
Clinical
Forensics/Toxicology
Therapeutic / Clinical
Therapeutic / Clinical
Therapeutic / Clinical

Applications

Nicotines at High pH using Kinetex 5u EVO C18 100x3.0mm
Analytes
13-OH-Cotinine
2Nornicotine
3Cotinine
4Anabasine
5Nicotine
6
Compound Name
Hydroxycotinine
CID
414
Molecular Formula
C10H12N2O2
Molecular Weight
192
No. Chiral Centers:
2
No. Hydrogen Bond Acceptors
3
No. Hydrogen Bond Donors
1
Details
LC Conditions (App ID: 22721)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
EVO C18
Elution Type:
Gradient
Mobile Phase:
A: 20 mM Ammonium bicarbonate pH 8.2
B: Methanol
Gradient Profile:
Step No.Time(min)%A%B
109010
231090
33.19010
459010
55955
Flow Rate:
0.75 mL/min
Temperature:
22°C
Detection:
Mass Spectrometer (MS) @ 254 amu (22°C)
Detector Info:
SCIEX API 4000™

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