Applications

ARG-Vasopressin Extracted Ion Chromatograms, QCL (0.500 ng/mL)

Analytes

1Oxytocin
2ARG-Vasopressin

Compound Name Endopituitrina
CID 5771
Molecular Formula C43H66N12O12S2
Molecular Weight 1007
No. Chiral Centers: 9
No. Hydrogen Bond Acceptors 13
No. Hydrogen Bond Donors 12

Details

LC Conditions (App ID: 22772)

Column:

Luna 3 µm PFP(2) 100 Å, LC Column 50 x 2 mm, Ea

Brand Name:

Luna

Part No:

00B-4447-B0

Phase Name:

PFP(2)

Elution Type:

Gradient

Mobile Phase:

A: 0.1%FA in water

B: 0.1%FA in 50:50 MeOH:Acetonitrile

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	90	10
2	0.2	90	10
3	0.5	70	30
4	3.5	70	30
5	3.51	30	70
6	4	30	70
7	4.01	90	10
8	5	90	10

Flow Rate:

0.45 mL/min

Temperature:

22°C

Detection:

Tandem Mass Spec (MS-MS) (40°C)

Detector Info:

SCIEX API 5000

Sample Preparation Details

Description: Strata-X-CW 33 µm Polymeric Weak Cation, 30 mg / well, 96-Well Plates, 2/Pk
Part Number: 8E-S035-TGB
Condition 1: Methanol
Condition 2: Water
Wash 1: 4% H3PO4 in water
Wash 2: 30% MeOH in water
Evaporation: Evaporate to dryness
Sample Notes:
1 Condition Methanol
2 Wash 30% MeOH in water
1 Shake 1% TFA in 50:50 MeOH: Acetonitrile
2 Wash 30% MeOH in water
1 Shake 1% TFA in 50:50 MeOH: Acetonitrile
2 Wash 30% MeOH in water

Order Items Used in This Application

00B-4447-B0

Luna 3 µm PFP(2) 100 Å, LC Column 50 x 2 mm, Ea

Qty:

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Similar Applications

Select Analyte of Interest

Oxytocin Extracted Ion Chromatograms, QCL (0.500 ng/mL)

Luna 3 µm PFP(2) 100 Å, LC Column 50 x 2 mm, Ea

Reversed Phase

Oxytocin and ARG-Vasopressin Chromatogram

Luna 3 µm PFP(2) 100 Å, LC Column 50 x 2 mm, Ea

Reversed Phase

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