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Applications
LCMS of Vitamin D epimers on Kinetex 2.6u F5 100x4.6mm
22810
Separation Mode: Reversed Phase
Reversed Phase
F5
HPLC
Vitamins
Clinical
Therapeutic / Clinical
Chemical Compound

Applications

LCMS of Vitamin D epimers on Kinetex 2.6u F5 100x4.6mm
Analytes
125-OH Vitamin D3
225-OH Vitamin D2
33-epi-25-OH Vitamin D3
Compound Name
Calcifediol
CID
5283731
Molecular Formula
C27H44O2
Molecular Weight
400
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
2
Details
LC Conditions (App ID: 22810)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
F5
Elution Type:
Isocratic
Mobile Phase:
A: Water with 0.1% formic acid
B: Methanol with 0.1% formic acid
Gradient Profile:
Step No.Time(min)%A%B
101585
Flow Rate:
0.75 mL/min
Temperature:
22°C
Detection:
Tandem Mass Spec (MS-MS) (22°C)

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