| 1Methyl paraben |
|---|
| 2Ethyl paraben |
| 3Propyl paraben |
| 4Butyl paraben |
Compound Name Methyl Paraben |
CID 7456 |
Molecular Formula C8H8O3 |
Molecular Weight 152 |
No. Hydrogen Bond Acceptors 3 |
No. Hydrogen Bond Donors 1 |
Applications
Extraction of Parabens from Baby Wash using Novum SLE 12cc Tube & Kinetex 5u EVO C18, 100x3.0
22848
Separation Mode: Reversed Phase
Reversed Phase
EVO C18
HPLC
Preservatives, Pharmaceutical
Personal Care/Consumer Products
Personal Healthcare / Cosmetic
Product / Trade Good
Applications
Extraction of Parabens from Baby Wash using Novum SLE 12cc Tube & Kinetex 5u EVO C18, 100x3.0
Analytes
Details
LC Conditions (App ID: 22848)
Column: | ||||||||||||||||||||
Brand Name: Kinetex | ||||||||||||||||||||
Part No: | ||||||||||||||||||||
Phase Name: EVO C18 | ||||||||||||||||||||
Elution Type: Gradient | ||||||||||||||||||||
Mobile Phase: A: 10% acetonitrile in water B: Acetonitrile | ||||||||||||||||||||
Gradient Profile:
| ||||||||||||||||||||
Flow Rate: 1.5 mL/min | ||||||||||||||||||||
Temperature: 22°C | ||||||||||||||||||||
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C) | ||||||||||||||||||||
Sample Note: Analytes
1. Methyl paraben
2. Ethyl paraben
3. Propyl paraben
4. Butyl paraben
Novum SLE 12cc Tube
8B-S138-KDG
Novum SLE 12cc Tube
Extraction Procedure
Sample pre-treatment
0.5 ± 0.05 g spiked Aveeno Baby (Wash & Shampoo) sample
Add 1.25 mL acetone
Add 0.6 ml 1% Nacl solution
Vortex for 30 secs
Wait 30 secs till the emulsion dissipates
Centrifuge for 10 mins at 3000 rpm
Collect the supernatant and proceed to load
Sample loading
"Load the sample from pre-treatment step above onto the Novum SLE cartridge and apply a short and gentle pulse of vacuum (~ 5" of Hg for 5-10 sec or until the sample has completely entered the media).
"Wait for 5 minutes.
Elution
"Dispense 10 mL of ethylacetate onto the SLE media and allow the solvent to elute by gravity for 10 mins and collect the eluant.
"Apply vacuum at 5"-10"of Hg to complete the extraction.
NOTE: Prolonged application of vacuum will result in elution of body wash out of the SLE media and into the final extracted solvent.
Dry down
"Evaporate the final extract to complete dryness under slow stream of N2 at 45°C
Reconstitute
"Reconstitute the dry residue in 0.5 mL of methanol
"Centrifuge for 5 mins at 6000 rpm
"Collect supernatant to shoot on HPLC |
Order Items Used in This Application
00D-4633-Y0
Kinetex 5 µm EVO C18 100 Å, LC Column 100 x 3.0 mm, Ea
Qty:
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Methyl paraben
Kinetex 5 µm EVO C18 100 Å, LC Column 100 x 3.0 mm, Ea
Reversed Phase
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