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Applications
Acetaminophen, Aspirin, and Caffeine - USP Method
3270
Separation Mode: Reversed Phase
Reversed Phase
USP L01
C18(2)
HPLC
Anti-Infective Agents
Pharmaceutical
Therapeutic / Clinical

Applications

Acetaminophen, Aspirin, and Caffeine - USP Method
Analytes
1Acetaminophen
2Caffeine
3Aspirin
4Benzoic acid
5Salicylic acid
Compound Name
Acetaminophen
CID
1983
Molecular Formula
C8H9NO2
Molecular Weight
151
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
2
Details
Official Method: USP L01
LC Conditions (App ID: 3270)
Column:
Brand Name:
Part No:
Phase Name:
C18(2)
Elution Type:
Isocratic
Mobile Phase:
A: water
B: methanol
C: glacial acetic acid
Gradient Profile:
Step No.Time(min)%A%B%C
1069283
Flow Rate:
2 mL/min
Temperature:
45°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 275 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282

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