| 1L-Ascorbic acid |
|---|
| 2Niacinamide |
| 3Pyridoxal |
| 4p-Aminobenzoic acid |
| 5Thiamine |
| 6Folic acid |
| 7Riboflavin |
Compound Name Ascorbic Acid |
CID 5785 |
Molecular Formula C6H8O6 |
Molecular Weight 176 |
No. Hydrogen Bond Acceptors 6 |
No. Hydrogen Bond Donors 4 |
Applications
Water-Soluble Vitamins Chromatographic Comparison
5482
Separation Mode: Reversed Phase
Reversed Phase
ODS-2
HPLC
Vitamin B Complex
Pharmaceutical
Therapeutic / Clinical
Analytes
Details
LC Conditions (App ID: 5482)
Column: | ||||||||||||
Brand Name: | ||||||||||||
Part No: | ||||||||||||
Phase Name: ODS-2 | ||||||||||||
Elution Type: Gradient | ||||||||||||
Mobile Phase: A: 5 mM Sodium 1-Pentanesulfonate; 0.1% H3PO4 in water B: 5 mM Sodium 1-Pentanesulfonate; 0.1% H3PO4 in 80% acetonitrile in water | ||||||||||||
Gradient Profile:
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Flow Rate: 1 mL/min | ||||||||||||
Temperature: 22°C | ||||||||||||
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C) | ||||||||||||
Sample Note: SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287
- Holder Part No.: KJ0-4282 |
Order Items Used in This Application
00F-3300-E0
Prodigy 5 µm ODS(2) 150 Å, LC Column 150 x 4.6 mm, Ea
Qty:
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L-Ascorbic acid
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