Erythromycins

LC Conditions

Column

PolymerX 7 µm RP-1 100 Å, LC Column 250 x 4.6 mm, Ea

Brand

PolymerX

Part No

00G-4327-E0

Phase Name

RP-1

Elution Type

Isocratic

Mobile Phase

A: Acetonitrile

B: K2HPO4 (1.75g), pH 9.0/Tertiary butyl alcohol/Acetonitrile/Water (50:135:30:755) (v/v/v/v)

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

50

50

Flow Rate

0.80 mL/min

Temperature

75°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, RP-1 4 x 3.0mm, 10/Pk Part No.: AJ0-5809 - Holder Part No.: KJ0-4282