Tetracyclines

LC Conditions

Column

PolymerX 5 µm RP-1 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

PolymerX

Part No

00F-4326-E0

Phase Name

RP-1

Elution Type

Isocratic

Mobile Phase

A: 50g Tertiary butyl alcohol in water

B: Phosphate buffer, pH 7.5

C: Tetrabutyl ammonium hydrogen sulfate

D: 10mL EDTA disodium solution in water

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

20

6

Flow Rate

1.00 mL/min

Temperature

60°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, RP-1 4 x 3.0mm, 10/Pk Part No.: AJ0-5809 - Holder Part No.: KJ0-4282