Diisocyanate

LC Conditions

Column

Luna 5 µm Phenyl-Hexyl 100 Å, LC Column 250 x 4.6 mm, Ea

Brand

Luna

Part No

00G-4257-E0

Phase Name

Phenyl-Hexyl

Elution Type

Gradient

Mobile Phase

A: 0.007M Ammonium acetate, pH 6.0/Acetonitrile (67.5:32.5 v/v)

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

0

2

5

100

0

3

20

75

25

4

30

65

35

5

35

100

0

6

40

100

0

Flow Rate

0.60 mL/min

Temperature

20°C

Detection

Fluorescence (FLUOR) @ (22°C)

Detection Info

Sample Notes

The derivatisation reagent used was 1-(2-pyridyl)piperazine (1-2PP). SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Phenyl 4 x 3.0mm, 10/Pk Part No.: AJ0-4351 - Holder Part No.: KJ0-4282