Nicotine and Related Compounds

LC Conditions

Column

Synergi 4 µm Max-RP 80 Å, LC Column 150 x 4.6 mm, Ea

Brand

Synergi

Part No

00F-4337-E0

Phase Name

Max-RP

Elution Type

Gradient

Mobile Phase

A: 10 mM Ammonium acetate in water

B: 10 mM Ammonium acetate in methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

0.25

90

10

3

3.75

0

100

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074 - Holder Part No.: KJ0-4282