Corticosteroids using Ammonium Hydroxide
LC Conditions
Column
Luna 3 µm C18(2) 100 Å, LC Column 30 x 4.6 mm, Ea
Brand
Luna
Part No
00A-4251-E0
Phase Name
C18(2)
Elution Type
Gradient
Mobile Phase
A: Water w/0.1% ammonium hydroxide, pH 10
B: Acetonitrile w/0.1% ammonium hydroxide, pH 10
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
85
15
2
3
25
75
Flow Rate
2.00 mL/min
Temperature
25°C
Detection
UV-Vis Abs.-Variable Wave.(UV) @ (22°C)
Detection Info
Sample Notes
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282
Order Items Used in This Application
- 1. Estriol
- 2. Estradiol
- 3. Estrone
-OEChem-06211003152D<br><br> 45 48 0 1 0 0 0 0 0999 V2000<br> 9.1857 2.1128 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.4586 0.3575 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.5357 -2.1948 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.9288 0.8575 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 7.9288 -0.1425 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 7.0628 -0.6425 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 6.1968 -0.1425 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 8.8750 1.1623 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 7.0628 1.3575 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.8750 -0.4472 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.4586 0.3575 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 6.1968 0.8575 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.0789 -1.6840 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.9288 1.8575 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.2868 -0.6493 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.1808 -2.2117 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.2787 -1.6909 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3599 -0.0852 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3433 -2.2406 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4120 -0.6132 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4037 -1.6981 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.0188 -0.9877 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.8022 -1.0618 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.9329 0.2825 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.4875 1.2585 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.4613 1.8325 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.6643 1.8325 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6240 -1.0141 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.4124 -0.7564 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.7404 0.9098 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.9847 1.4401 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.5862 0.7499 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.2969 -2.2644 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.6880 -1.5685 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.5488 1.8575 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.9288 2.4775 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.3088 1.8575 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.7835 -2.6877 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.5818 -2.6846 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.7924 2.2406 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.7687 -0.1794 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3719 0.5347 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3457 -2.8606 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8787 -0.2970 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -1.8827 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8 1 1 1 0 0 0<br> 1 40 1 0 0 0 0<br> 11 2 1 6 0 0 0<br> 2 41 1 0 0 0 0<br> 3 21 1 0 0 0 0<br> 3 45 1 0 0 0 0<br> 4 5 1 0 0 0 0<br> 4 8 1 0 0 0 0<br> 4 9 1 0 0 0 0<br> 4 14 1 1 0 0 0<br> 5 6 1 0 0 0 0<br> 5 10 1 0 0 0 0<br> 5 22 1 6 0 0 0<br> 6 7 1 0 0 0 0<br> 6 13 1 0 0 0 0<br> 6 23 1 1 0 0 0<br> 7 12 1 0 0 0 0<br> 7 15 1 0 0 0 0<br> 7 24 1 6 0 0 0<br> 8 11 1 0 0 0 0<br> 8 25 1 0 0 0 0<br> 9 12 1 0 0 0 0<br> 9 26 1 0 0 0 0<br> 9 27 1 0 0 0 0<br> 10 11 1 0 0 0 0<br> 10 28 1 0 0 0 0<br> 10 29 1 0 0 0 0<br> 11 30 1 0 0 0 0<br> 12 31 1 0 0 0 0<br> 12 32 1 0 0 0 0<br> 13 16 1 0 0 0 0<br> 13 33 1 0 0 0 0<br> 13 34 1 0 0 0 0<br> 14 35 1 0 0 0 0<br> 14 36 1 0 0 0 0<br> 14 37 1 0 0 0 0<br> 15 17 2 0 0 0 0<br> 15 18 1 0 0 0 0<br> 16 17 1 0 0 0 0<br> 16 38 1 0 0 0 0<br> 16 39 1 0 0 0 0<br> 17 19 1 0 0 0 0<br> 18 20 2 0 0 0 0<br> 18 42 1 0 0 0 0<br> 19 21 2 0 0 0 0<br> 19 43 1 0 0 0 0<br> 20 21 1 0 0 0 0<br> 20 44 1 0 0 0 0<br>M END
Compound Name
Estriol
CID
5756
Molecular Formula
C18H24O3
Molecular Weight
288
No. Hydrogen Bond Acceptors
3
No. Hydrogen Bond Donors
3
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