Black Cohosh Root (Cimicifuga racemosa)
LC Conditions
Column
Prodigy 5 µm ODS-3 100 Å, LC Column 150 x 4.6 mm, Ea
Brand
Prodigy
Part No
00F-4097-E0
Phase Name
ODS-3
Elution Type
Gradient
Mobile Phase
A: Water
B: Acetonitrile
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
75
25
2
30
25
75
3
30
30
70
4
33
0
100
5
45
80
20
Flow Rate
1.30 mL/min
Temperature
25°C
Detection
Electrochemical (ELEC) @ (22°C)
Detection Info
Sample Notes
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282
Order Items Used in This Application
- 1. Actein
- 2. 27-Deoxyactein
- 3. Cimicidiol
- 4. Cimicifugoside
-OEChem-05151001542D<br><br> 98106 0 1 0 0 0 0 0999 V2000<br> 13.9809 -0.5298 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0022 2.0967 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 14.9463 -2.3676 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 15.8973 -0.1582 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 16.6732 -2.8074 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1304 0.6000 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2740 3.1033 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.5381 2.1100 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.5304 0.1100 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.5323 0.0725 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 8.6663 0.5725 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 8.6660 -0.4279 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.3984 0.5725 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 11.3084 0.0657 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 8.6663 1.5725 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 11.3164 -0.9759 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 9.5163 -0.9690 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.7724 0.0379 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.3984 1.5725 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.4143 -1.4967 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.5323 2.0725 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.7724 2.1072 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.3044 -1.2857 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 12.2915 0.3907 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.9058 -0.4428 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 6.8663 0.5517 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.8663 1.5933 0.0000 C 0 0 3 0 0 0 0 0 0 0 0 0<br> 11.3006 1.0656 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.3241 -1.9759 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.7364 -2.2740 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 7.2824 2.9789 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.2823 2.9674 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 14.4356 -1.5079 0.0000 C 0 0 3 0 0 0 0 0 0 0 0 0<br> 13.8091 -2.3858 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 15.0954 -0.7565 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 12.1462 -3.0813 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 16.0140 -1.1518 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 15.9218 -2.1476 0.0000 C 0 0 3 0 0 0 0 0 0 0 0 0<br> 5.1343 1.6000 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 16.9325 -1.5472 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2702 2.1034 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 3.4022 1.6067 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 4.2625 0.1034 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3984 0.6067 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 8.0555 -0.5355 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.8782 -1.0105 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.1312 1.0032 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6729 2.4225 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.9071 -0.8535 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.2982 -1.5494 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.3787 -0.4412 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.1770 -0.4319 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.0089 1.4649 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.6104 2.1551 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.0133 -1.9696 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.8116 -1.9727 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.9309 2.5475 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.1338 2.5475 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.7367 -1.9184 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.8265 0.7040 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.0361 0.9556 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 13.3194 0.2997 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.2554 0.6578 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.6572 -0.0320 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.8712 2.4433 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.9206 1.0704 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.2959 1.6856 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.6807 1.0609 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.7041 -1.9807 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.3289 -2.5959 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.9441 -1.9711 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.1201 -2.2062 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.8229 3.2827 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.9786 3.5194 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.7419 2.6752 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.8157 2.6512 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.5985 3.5007 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.7490 3.2836 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 13.6403 -2.9824 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 14.3680 -2.6543 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 14.5188 -0.1320 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.6457 -2.7154 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.7802 -3.5818 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.6467 -3.4472 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 15.7120 -2.9713 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1367 2.2200 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 16.6874 -2.1166 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 17.5020 -1.7923 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 17.1776 -0.9777 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7344 2.4154 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 16.5518 -3.4154 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4046 2.2267 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.8621 -0.3700 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.6592 -0.3731 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8626 0.9188 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7383 3.4154 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 1.8021 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.5280 -0.5100 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 25 1 0 0 0 0<br> 1 33 1 0 0 0 0<br> 2 27 1 0 0 0 0<br> 39 2 1 6 0 0 0<br> 3 33 1 0 0 0 0<br> 3 38 1 0 0 0 0<br> 4 35 1 0 0 0 0<br> 4 37 1 0 0 0 0<br> 5 38 1 0 0 0 0<br> 5 91 1 0 0 0 0<br> 6 39 1 0 0 0 0<br> 6 43 1 0 0 0 0<br> 41 7 1 1 0 0 0<br> 7 96 1 0 0 0 0<br> 42 8 1 6 0 0 0<br> 8 97 1 0 0 0 0<br> 44 9 1 1 0 0 0<br> 9 98 1 0 0 0 0<br> 10 11 1 0 0 0 0<br> 10 12 1 6 0 0 0<br> 10 13 1 0 0 0 0<br> 10 17 1 0 0 0 0<br> 11 12 1 6 0 0 0<br> 11 15 1 0 0 0 0<br> 11 18 1 0 0 0 0<br> 12 45 1 0 0 0 0<br> 12 46 1 0 0 0 0<br> 13 14 1 0 0 0 0<br> 13 19 1 0 0 0 0<br> 13 47 1 6 0 0 0<br> 14 16 1 0 0 0 0<br> 14 24 1 0 0 0 0<br> 14 28 1 1 0 0 0<br> 15 21 1 0 0 0 0<br> 15 22 1 0 0 0 0<br> 15 48 1 1 0 0 0<br> 16 20 1 0 0 0 0<br> 16 23 1 0 0 0 0<br> 16 29 1 6 0 0 0<br> 17 20 1 0 0 0 0<br> 17 49 1 0 0 0 0<br> 17 50 1 0 0 0 0<br> 18 26 1 0 0 0 0<br> 18 51 1 0 0 0 0<br> 18 52 1 0 0 0 0<br> 19 21 1 0 0 0 0<br> 19 53 1 0 0 0 0<br> 19 54 1 0 0 0 0<br> 20 55 1 0 0 0 0<br> 20 56 1 0 0 0 0<br> 21 57 1 0 0 0 0<br> 21 58 1 0 0 0 0<br> 22 27 1 0 0 0 0<br> 22 31 1 0 0 0 0<br> 22 32 1 0 0 0 0<br> 23 25 1 0 0 0 0<br> 23 30 1 0 0 0 0<br> 23 59 1 1 0 0 0<br> 24 25 1 0 0 0 0<br> 24 60 1 0 0 0 0<br> 24 61 1 0 0 0 0<br> 25 62 1 1 0 0 0<br> 26 27 1 0 0 0 0<br> 26 63 1 0 0 0 0<br> 26 64 1 0 0 0 0<br> 27 65 1 0 0 0 0<br> 28 66 1 0 0 0 0<br> 28 67 1 0 0 0 0<br> 28 68 1 0 0 0 0<br> 29 69 1 0 0 0 0<br> 29 70 1 0 0 0 0<br> 29 71 1 0 0 0 0<br> 30 34 1 0 0 0 0<br> 30 36 1 1 0 0 0<br> 30 72 1 0 0 0 0<br> 31 73 1 0 0 0 0<br> 31 74 1 0 0 0 0<br> 31 75 1 0 0 0 0<br> 32 76 1 0 0 0 0<br> 32 77 1 0 0 0 0<br> 32 78 1 0 0 0 0<br> 33 34 1 0 0 0 0<br> 33 35 1 0 0 0 0<br> 34 79 1 0 0 0 0<br> 34 80 1 0 0 0 0<br> 35 37 1 0 0 0 0<br> 35 81 1 6 0 0 0<br> 36 82 1 0 0 0 0<br> 36 83 1 0 0 0 0<br> 36 84 1 0 0 0 0<br> 37 38 1 0 0 0 0<br> 37 40 1 6 0 0 0<br> 38 85 1 0 0 0 0<br> 39 41 1 0 0 0 0<br> 39 86 1 0 0 0 0<br> 40 87 1 0 0 0 0<br> 40 88 1 0 0 0 0<br> 40 89 1 0 0 0 0<br> 41 42 1 0 0 0 0<br> 41 90 1 0 0 0 0<br> 42 44 1 0 0 0 0<br> 42 92 1 0 0 0 0<br> 43 44 1 0 0 0 0<br> 43 93 1 0 0 0 0<br> 43 94 1 0 0 0 0<br> 44 95 1 0 0 0 0<br>M END
Compound Name
Actein
CID
6537491
Molecular Formula
C35H54O9
Molecular Weight
618
No. Hydrogen Bond Acceptors
9
No. Hydrogen Bond Donors
4
Similar Applications
Reversed Phase
Black Cohosh Root (Cimicifuga racemosa)
Prodigy 5 µm ODS-3 100 Å, LC Column 150 x 4.6 mm, Ea
1112