Sennosides

LC Conditions

Column

Luna 5 µm Phenyl-Hexyl 100 Å, LC Column 250 x 4.6 mm, Ea

Brand

Luna

Part No

00G-4257-E0

Phase Name

Phenyl-Hexyl

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA in water

B: 0.1% TFA in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

84

16

2

45

80

20

3

48

10

90

4

54

10

90

5

55

84

16

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Phenyl 4 x 3.0mm, 10/Pk Part No.: AJ0-4351 - Holder Part No.: KJ0-4282