Soy Isoflavones - HPLC
LC Conditions
Column
Luna 5 µm C18(2) 100 Å, LC Column 250 x 4.6 mm, Ea
Brand
Luna
Part No
00G-4252-E0
Phase Name
C18(2)
Elution Type
Gradient
Mobile Phase
A: Water + 0.1% Phosphoric
B: Methanol
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
72
28
2
2
63
37
3
30
27
73
Flow Rate
1.00 mL/min
Temperature
35°C
Detection
UV-Vis Abs.-Variable Wave.(UV) @ (35°C)
Detection Info
Sample Notes
Application courtesy of AM Todd Botanicals
Order Items Used in This Application
- 1. Daidzin
- 2. Glycitin
- 3. Genistin
- 4. Malonyl diadzin
- 5. Malonyl glycitin
- 6. Acetyl daidin
- 7. Malonyl genistin
- 8. Daidzein
- 9. Glycitein
- 10. Genistein
-OEChem-05271007422D<br><br> 50 53 0 1 0 0 0 0 0999 V2000<br> 12.1292 1.1060 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.3972 1.0994 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.1408 -1.8939 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 13.8690 -0.8873 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.4049 -0.9006 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 13.8574 2.1127 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.8671 1.0752 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.8671 -1.9248 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.5369 -2.4248 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.1369 -0.8939 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 13.0010 -0.3906 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 11.2690 -0.3973 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 12.9972 0.6094 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 11.2651 0.6027 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 13.8613 1.1127 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.5331 0.5960 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6270 1.1099 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.5331 -0.4456 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.7331 0.5752 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.7331 -0.4248 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6270 -0.9594 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.8671 -0.9248 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0010 -0.4248 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0010 0.5752 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1350 -0.9248 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1350 -1.9248 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2690 -0.4248 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2690 -2.4248 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4030 -0.9248 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4030 -1.9248 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.6012 -1.2060 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 13.0034 -1.0106 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.2714 -1.0173 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.9948 1.2294 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.7294 0.2906 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 14.0756 0.5309 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 14.4714 1.2227 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.6789 -2.2019 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 14.4047 -0.5752 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.4073 -1.5206 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 14.3931 2.4248 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6199 1.7298 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.0688 -0.7577 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6199 -1.5794 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.4641 0.8852 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.6720 -2.2348 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2690 0.1952 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2690 -3.0448 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 -0.6148 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -2.1148 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 13 1 0 0 0 0<br> 1 14 1 0 0 0 0<br> 14 2 1 6 0 0 0<br> 2 16 1 0 0 0 0<br> 10 3 1 6 0 0 0<br> 3 38 1 0 0 0 0<br> 11 4 1 1 0 0 0<br> 4 39 1 0 0 0 0<br> 12 5 1 1 0 0 0<br> 5 40 1 0 0 0 0<br> 6 15 1 0 0 0 0<br> 6 41 1 0 0 0 0<br> 7 19 1 0 0 0 0<br> 7 24 1 0 0 0 0<br> 8 22 2 0 0 0 0<br> 9 30 1 0 0 0 0<br> 9 50 1 0 0 0 0<br> 10 11 1 0 0 0 0<br> 10 12 1 0 0 0 0<br> 10 31 1 0 0 0 0<br> 11 13 1 0 0 0 0<br> 11 32 1 0 0 0 0<br> 12 14 1 0 0 0 0<br> 12 33 1 0 0 0 0<br> 13 15 1 6 0 0 0<br> 13 34 1 0 0 0 0<br> 14 35 1 0 0 0 0<br> 15 36 1 0 0 0 0<br> 15 37 1 0 0 0 0<br> 16 17 2 0 0 0 0<br> 16 18 1 0 0 0 0<br> 17 19 1 0 0 0 0<br> 17 42 1 0 0 0 0<br> 18 21 2 0 0 0 0<br> 18 43 1 0 0 0 0<br> 19 20 2 0 0 0 0<br> 20 21 1 0 0 0 0<br> 20 22 1 0 0 0 0<br> 21 44 1 0 0 0 0<br> 22 23 1 0 0 0 0<br> 23 24 2 0 0 0 0<br> 23 25 1 0 0 0 0<br> 24 45 1 0 0 0 0<br> 25 26 2 0 0 0 0<br> 25 27 1 0 0 0 0<br> 26 28 1 0 0 0 0<br> 26 46 1 0 0 0 0<br> 27 29 2 0 0 0 0<br> 27 47 1 0 0 0 0<br> 28 30 2 0 0 0 0<br> 28 48 1 0 0 0 0<br> 29 30 1 0 0 0 0<br> 29 49 1 0 0 0 0<br>M END
Compound Name
Daidzin
CID
107971
Molecular Formula
C21H20O9
Molecular Weight
416
No. Hydrogen Bond Acceptors
9
No. Hydrogen Bond Donors
5
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