Protein on Jupiter C4 in 5 mM TFA

LC Conditions

Column

Jupiter 5 µm C4 300 Å, LC Column 250 x 4.6 mm, Ea

Brand

Jupiter

Part No

00G-4167-E0

Phase Name

C4

Elution Type

Gradient

Mobile Phase

A: Water with 5mM TFA

B: Acetonitrile with 5mM TFA

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

75

25

2

20

5

95

Flow Rate

1.00 mL/min

Temperature

35°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Widepore C4 4 x 3.0mm, 10/Pk Part No.: AJ0-4330 - Holder Part No.: KJ0-4282