Basic peptides on Jupiter C4 in 1.25mM TFA

LC Conditions

Column

Jupiter 5 µm C18 300 Å, LC Column 250 x 4.6 mm, Ea

Brand

Jupiter

Part No

00G-4053-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: Water with 1.25mM TFA

B: Acetonitrile with 1.25mM TFA

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

20

55

45

Flow Rate

1.00 mL/min

Temperature

35°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Widepore C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4321 - Holder Part No.: KJ0-4282