Methylene Selectivity on Jupiter C18 using Peptide Standards

LC Conditions

Column

Jupiter 5 µm C18 300 Å, LC Column 250 x 4.6 mm, Ea

Brand

Jupiter

Part No

00G-4053-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA

B: 0.085% TFA in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

20

55

45

Flow Rate

20.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Widepore C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4321 - Holder Part No.: KJ0-4282